Phosphoproteomics: a valuable tool for uncovering molecular signaling in cancer cells.

TitlePhosphoproteomics: a valuable tool for uncovering molecular signaling in cancer cells.
Publication TypeJournal Article
Year of Publication2021
AuthorsGerritsen JS, White FM
JournalExpert Rev Proteomics
Date Published2021 Aug

INTRODUCTION: Many pathologies, including cancer, have been associated with aberrant phosphorylation-mediated signaling networks that drive altered cell proliferation, migration, metabolic regulation, and can lead to systemic inflammation. Phosphoproteomics, the large-scale analysis of protein phosphorylation sites, has emerged as a powerful tool to define signaling network regulation and dysregulation in normal and pathological conditions.

AREAS COVERED: We provide an overview of methodology for global phosphoproteomics as well as enrichment of specific subsets of the phosphoproteome, including phosphotyrosine and phospho-motif enrichment of kinase substrates. We review quantitative methods, advantages and limitations of different mass spectrometry acquisition formats, and computational approaches to extract biological insight from phosphoproteomics data. Throughout, we discuss various applications and their challenges in implementation.

EXPERT OPINION: Over the past 20 years the field of phosphoproteomics has advanced to enable deep biological and clinical insight through the quantitative analysis of signaling networks. Future areas of development include Clinical Laboratory Improvement Amendments (CLIA)-approved methods for analysis of clinical samples, continued improvements in sensitivity to enable analysis of small numbers of rare cells and tissue microarrays, and computational methods to integrate data resulting from multiple systems-level quantitative analytical methods.

Alternate JournalExpert Rev Proteomics
PubMed ID34468274
PubMed Central IDPMC8628306
Grant ListR01 GM139998 / GM / NIGMS NIH HHS / United States
U01 CA215709 / CA / NCI NIH HHS / United States
U01 CA238720 / CA / NCI NIH HHS / United States
U54 CA210180 / CA / NCI NIH HHS / United States