Title | Rapid MALDI mass spectrometry imaging for surgical pathology. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Basu SS, Regan MS, Randall EC, Abdelmoula WM, Clark AR, Lopez BGimenez-Ca, Cornett DS, Haase A, Santagata S, Agar NYR |
Journal | NPJ Precis Oncol |
Volume | 3 |
Pagination | 17 |
Date Published | 2019 |
ISSN | 2397-768X |
Abstract | Matrix assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is an emerging analytical technique, which generates spatially resolved proteomic and metabolomic images from tissue specimens. Conventional MALDI MSI processing and data acquisition can take over 30 min, limiting its clinical utility for intraoperative diagnostics. We present a rapid MALDI MSI method, completed under 5 min, including sample preparation and analysis, providing a workflow compatible with the clinical frozen section procedure. |
DOI | 10.1038/s41698-019-0089-y |
Alternate Journal | NPJ Precis Oncol |
PubMed ID | 31286061 |
PubMed Central ID | PMC6609678 |
Grant List | R01 CA201469 / CA / NCI NIH HHS / United States T32 HL007627 / HL / NHLBI NIH HHS / United States P41 EB015898 / EB / NIBIB NIH HHS / United States R25 CA089017 / CA / NCI NIH HHS / United States U54 CA210180 / CA / NCI NIH HHS / United States |